Resources

Software

Low Biomass Background Correction (LBBC), an R package to denoise metagenomic cell-free DNA sequencing data is available on Github. The principles implemented in LBBC are described in detail here.

Protocols

A step-by-step protocol for Droplet Assisted RNA Targeting by single cell sequencing (DART-seq) is available at Protocols Exchange. DART-seq allows simultaneous multiplexed amplicon sequencing and transcriptome analysis in single cells.

A step-by-step protocol of the single-standed DNA libary preparation we use to sequence cell-free DNA isolated from plasma and urine, can be downloaded here. This libary preparation is well suited for the recovery and DNA sequencing of ultrashort, highly fragmented cell-free DNA, as we describe here.