HiPR-FISH (high-phylogenetic-resolution microbiome mapping by fluorescence in situ hybridization) enables automated image analysis for highly multiplexed FISH experiments on microbial communities. The details are described in this manuscript.
DART-seq (Droplet-Assisted RNA Targeting by single-cell sequencing) is a protocol that enables simultaneous multiplexed amplicon sequencing and transcriptome analysis in single cells. More information can be found in this manuscript.
ssDNA ligation is a step-by-step protocol of the single-stranded DNA library preparation we use to sequence cell-free DNA isolated from plasma and urine. This library preparation is well suited for the recovery and DNA sequencing of ultrashort, highly fragmented cell-free DNA, as we describe in this manuscript.